Uv Vis Spectrophotometer

About Uv Vis Spectrophotometer

Ultraviolet-visible spectroscopy or ultraviolet-visible spectrophotometry (UV-Vis or UV/Vis) refers to absorption spectroscopy in the ultraviolet-visible spectral region. This means it uses light in the visible and adjacent (near-UV and near-infrared (NIR)) ranges. UV/Vis spectrophotometer is used in the quantitative determination of concentrations of the absorber in the solutions of transition metal ions and highly conjugated organic compounds.

UV/Vis spectroscopy can be used to determine the concentration of the absorber in a solution. The absorbance changes with concentration. This can be taken from references (tables of molar extinction coefficients), or more accurately, determined from a calibration curve.