About Fluorescence Microscope
A fluorescence microscope is an optical microscope that uses fluorescence and phosphorescence instead of, or in addition to, reflection and absorption to study properties of organic or inorganic substances.
Principle: The specimen is illuminated with light of a specific wavelength (or wavelengths) which is absorbed by the fluorophores, causing them to emit light of longer wavelengths (i.e., of a different color than the absorbed light). The illumination light is separated from the much weaker emitted fluorescence through the use of a spectral emission filter. Typical components of a fluorescence microscope are a light source (xenon arc lamp or mercury-vapor lamp), the excitation filter, the dichroic mirror (or dichroic beamsplitter), and the emission filter (see figure below). The filters and the dichroic are chosen to match the spectral excitation and emission characteristics of the fluorophore used to label the specimen. In this manner, the distribution of a single fluorophore (color) is imaged at a time. Multi-color images of several types of fluorophores must be composed by combining several single-color images
Optimized for Biological Research and DiagnosticsEngineered for laboratory, biological, and medical diagnostic use, this fluorescence microscope combines high sensitivity with advanced optics for reliable, high-accuracy imaging. The wide field eyepiece and range of objective lenses provide adaptability for diverse sample analyses, making it invaluable in research and clinical environments.
Flexible Illumination and Filter OptionsEquipped with an LED or mercury lamp with variable intensity, this microscope supports multiple filter sets, including blue, green, and UV filters, for both fluorescence and brightfield observation. These features facilitate superior sample visualization and enable users to tailor contrast and brightness according to experimental needs.
Durable, User-friendly DesignBuilt from high-grade metal and plastic, the microscope features a coaxial mechanical stage with a measurement scale for precise slide positioning. Manual coarse and fine focus mechanisms offer improved user control, while the upright stand and rectangular base provide stability in various environments.
FAQs of Fluorescence Microscope:
Q: How do I adjust the focus on this fluorescence microscope?
A: The microscope is equipped with both coarse and fine focus adjustment knobs. Use the coarse focus for general positioning and the fine focus for crisp, high-detail observation, ensuring precise focusing for delicate biological samples.
Q: What are the benefits of multiple objective lenses, ranging from 4X to 100X (oil immersion)?
A: Having multiple objective lenses allows you to switch between different magnification levels quickly, catering to both general overviews and detailed cellular analysis. The 100X oil immersion lens is particularly useful for observing minute structures with high resolution.
Q: When should I use the fluorescent filters (blue, green, UV) during observation?
A: Use fluorescent filters when examining samples labeled with specific fluorescent dyes. The blue, green, and UV filters enhance contrast and fluorescence emission, making target structures more visible under the corresponding excitation conditions.
Q: Where can this microscope be utilized effectively?
A: This microscope is ideal for laboratory settings, biological research institutes, clinical diagnostics, and educational environments across India. Its high sensitivity and versatility make it a valuable instrument for scientific, medical, and academic applications.
Q: What is the process for connecting a digital camera for imaging?
A: The microscope is compatible with digital camera adapters. Attach your camera using the appropriate adapter to the viewing tube for high-resolution image capture and documentation of your observations.
Q: How does the variable intensity light source enhance microscope usage?
A: Variable intensity from the LED or mercury lamp allows users to adjust illumination based on sample type and desired imaging mode, minimizing photobleaching and optimizing visualization for both fluorescence and brightfield analyses.
Q: What type of warranty is provided with this fluorescence microscope?
A: This equipment comes with a 1-year warranty, providing support and coverage on manufacturing defects or operational issues within the warranty period.